MOLECULAR IDENTIFICATION OF STREPTOMYCES PHAEOPURPUREUS ISOLATED FROM MARINE SEDIMENTS, VISAKHAPATNAM, USING 16S RRNA GENE SEQUENCE ANALYSIS

In order to obtain different actinomycetes isolates from the Visakhapatnam marine soils, an isolation study was carried out by using different growth media. A molecular taxonomic method, 16S rDNA gene sequencing, was applied to obtain high virulent isolate (DHB-603) isolated from Visakhapatnam marine soils. Phylogenetic dendrograms based on 16S rDNA nucleotide sequences were constructed by using neighbour-joining algorithm and maximum likelihood methods. In the light of phylogenetic analyses, it was determined that DHB-603 showed 99.5% similarity with Streptomyces phaeopurpureus, and in phylogenetic tree also DHB-603 and Streptomyces phaeopurpureus were in the same branch. Soil isolate obtained from this study contribute to taxonomy of Streptomyces and also many species will emerge.