ESTIMATION OF DIFFERENT EXTRACTS OF AMARANTHUS VIRIDIS L FOR INVITRO ANTIOXIDANT ACTIVITY

Back ground: The plant Amaranthus viridis L (Amaranthaceous) is commonly known as “Never-fading flower” and “Slender amaranth” in Greek and English respectively. These plants have been traditionally used as antiulcer, antileprotic, antiviral and bronchodilator for treatment of asthma. As there has been an upsurge of interest in the therapeutic potential medicinal plants as antioxidant, hence, the aim of present investigation was to discern evidence if Ayurveda remedy can be worthy for providing exogenous antioxidant as diet. Objective: Estimation of different extracts of Amaranthus viridis L for invitro antioxidant activity. Material and methods:The invitro antioxidant activity of aqueous, ethanol and petroleum ether extracts of Amaranthus viridis L was appraised by diphenyl picryl hydrazyl scavenging, nitric oxide scavenging, hydrogen peroxide scavenging and reducing power using standard procedure. The IC50 was determined. Results: Antioxidant activity of all extracts of Amaranthus viridis L was proved by declining in nitric oxide activity, hydrogen peroxide activity and diphenyl picryl hydrazyl free radical activity and also increasing in reducing power percentage. The aqueous extract of Amaranthus viridis L was found to be more effective than other extracts. Fifty percentage of inhibition concentration of aqueous extracts of Amaranthus viridis L was discerned to be 25.33μg/ml, 29.25 μg/ml, 35.17 μg/ml and 356.65 μg/ml for reducing power, diphenyl picryl hydrazyl free radical activity, nitric oxide activity and hydrogen peroxide activity respectively. Conclusion: This investigation advocates that aqueous, ethanol and petroleum ether extracts of Amaranthus viridis L manifest potential antioxidant activity which can be considered as nutrient supplement. Keywords: Amaranthus viridis L, invitro antioxidant activity, nutrient supplement.