Endogenous Cytokinins Dynamics in Mycelial Biomass of Basidiomycetes at Different Stages of Cultivation

The study of cytokininis content dynamics in micelial biomass of two Basidiomycetes species (Hericium coralloides, strain 2332, Fomitopsis officinalіs, strain 5004) at the different stages of cultivation was carried out by HPLC-MS. The maximum content of trans-zeatin, trans-zeatin riboside, isopentenyladenosine and isopentenyladenine in H. coralloides 2332 was shown to take place at the period of the most intensive growth whereas the development inhibition is accompanied by a decrease in free cytokinins levels. The free cytokinins levels in F. officinalis 5004 raised when no sharp fluctuations in growth parameters of mycelial biomass occurred. Changes in all cytokinins content during mycelia biomass growth were specific and indicate the involvement of these phytohormones in the regulation of growth and development of the studied mushrooms species.