Cytotoxicity Brine Shrimp Activity of Leptadenia Hastata (PER) Decne Leaves, Stem-Bark and Root Extract

Objective: The present study was conducted to test for in vivo Brine Shrimp Cytotocity activity of Leptadenia hastata leaves, stem-bark and root extracts after successive maceration in five solvents (n-hexane, dichloromethane (DCM) ethyl acetate chloroform and methanol) and correlate cytotoxicity results with known pharmacological activities of the plant. Methods: Cytotoxicity was evaluated in terms of LC50 (lethality concentration), 10 nauplii were added into three replicates of each concentration of the plant extracts, and after 24 h the surviving brine shrimp larvae were counted, and LC50was assessed. Results: Potent cytotoxicity was found for both the leaves, stem-bark and root extracts of Leptadenia hastata, results showed a concentration dependent increment in mortality rate of the brine shrimp nauplii and the n-hexane dichloromethane ethyl acetate, chloroform and methanol fractions of the leaves, stem-bark and root extracts were potent against the brine shrimp, with chloroform Leaf extract having the highest 3460.00 and Methanol as the lowest LC50 with 651.292. Conclusion: The result indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine.