Anticancer Evaluation of Secondary Metabolites in Hep- 2 Cell Lines from Exiguobacterium Profundum BC2-11

The stressed environment contains full of oxidative stress which constitutes the plants and microbes. These sustains in this environment by changing its biological activity by producing some of the metabolites as intermediate products to survive within the microbial population. As these produce different metabolites with different biological activities so this current investigation has focused on the characterization and biological evaluation of the potential metabolite from E. profundum BC2-11 which was isolated from the stressed environment. The crude pigment extract was subjected to HPTLC profile and it exhibited six peaks, the 5th peak was observed to be highest area of 5973.3 (47.39%) which was the bioactive metabolite. The crude colored metabolite was assessed for antimicrobial assessment against clinical pathogens and it revealed highest activity against maximum pathogens. The radical scavenging activity for the colored metabolite was determined through DPPH, metal chelation, reductive power and total antioxidant capacity. The colored metabolite disclosed 50% of inhibitory activity at lower concentrations. The in vitro cytotoxicity evaluation was analyzed in Hep-2 cell lines and the dilution of 1:4 indicated about 54% cell viability and this was confirmed through MTT assay. Further the colored metabolite will be taken for bioprospecting studies and pharmaceutical applications.