Induced Breeding of Striped Spiny Eel, Mastacembelus Pancalus: Considering Various Doses of Pituitary Gland Hormone

The induced breeding of the striped spiny eel Mastacembelus pancalus using PG hormone was done to develop breeding protocol of the species and the optimization of doses. The present study consisted of two trials and each trial had three treatments (T1, T2 and T3) with three replications of each. In first trial (double dose) fishes were injected with two separate doses of pituitary gland (PG) hormone. In the preliminary dose, females were injected at 55, 60 and 65 mg·kg-1 and in resolving dose, injected 105, 120 and 135 mg·kg-1 body weight in T1, T2 and T3 respectively. In second trial (single dose) fishes were injected only once at 160, 180 and 200 mg·kg-1 body weight in T1, T2 and T3 respectively. In both trials, males were injected at 55, 60 and 65 mg·kg-1 body weight in T1, T2 and T3 respectively. The water parameters such as water temperature, pH and DO were ranged from 26.5 to 31.2ºC, 7.06 to 8.04 and 4.1 to 5.6 mg·L-1 respectively which was not significantly (p<0.05) differ in different treatments in both trials. It is showed the male start to chase with female after 9-10 h of hormone injection and spawning take place after 7-9 h of chasing. It is showed that there were no significant differences in respect of spawning and incubation period in two trials and fishes were spawned 100% in all treatments. Spawning time and incubation period were ranged from 15 to 20 h and 36 to 41 h respectively in both trials. The treatment 2 of 1st trial showed significantly (p<0.05) higher performance in terms of fertilization rate (73.33%) and hatching rate (67.85%) than other treatments which suggested that PG dose (1st dose 60 mg·kg-1 + 2nd dose 120 mg·kg-1 body weight) could be better for inducing of M. pancalus.