PCR Detection of Botryosphaeria rhodina as a Biotic Elicitor in Enhancement of Trans-Resveratrol and Trans-Piceid Biosynthesis of Peanut Kernels during Germination

A fungus involved in enhancement of trans-resveratrol and trans-piceid biosynthesis of peanut kernels during germination was isolated and molecularly identified by PCR as Botryosphaeria rhodina. When peanut kernels were surface-disinfected, 2-d incubated for germination and artificial colonized with the isolated B. rhodina at 20 or 30oC for 96 h, trans-resveratrol and trans-piceid contents of the kernels increased substantially with time of artificial colonization. The interaction of B. rhodina with peanut kernels during germination in elicitation of resveratrol and piceid biosynthesis is obvious. For development of a rapid PCR procedure in screening B. rhodina isolates, which are widespread in nature, a specific primer set was designed based on the internal transcribed spacer (ITS) region of ribosomal DNA and used for PCR detection of the morphology-presumptive isolates of B. rhodina, a targeted 424-bp fragment was exclusively detected for the B. rhodina isolates. The fragment was also detected when the genomic DNA's extracted from peanut kernels after colonized with B. rhodina were used as template for PCR amplification. The quantity of the amplified fragment increased with an increase of time of colonization.