In-vitro Study on α-amylase Inhibitory Activity of Selected Ethnobotanical Plant Extracts and its Herbal Formulations

Background: Chronic disorder of carbohydrate, fat and protein metabolism characterized by increased fasting and postprandial blood sugar levels. Treatment of disorders in carbohydrate uptake required the inhibition of α-amylase plays a role in digestion of starch and glycogen, is considered a strategy for control the diabetes. Medicinal plants are an important source of phytochemical constituents with potential for inhibition of α-amylase and can be used as control of the diabetes. Objective: The present study deals with the evaluation of ethnomedicinal plants and their formulation for inhibitory activity on α-amylase enzyme. Material and Methods: The selected ethnobotanical plants of Gymnemasylvsetre, Cocciniagrandis, Tinosporacordifolia, Phyllanthus emblica, Aegelmarmelosusing the solvent of methanol, hexane, chloroform and ethyl acetate extraction and different herbal formulation possess significant inhibitory effect. Inhibitory effect further confirmation of glucose uptake by yeast cell and starch iodine assay of herbal formulation. Results: The formulation no. 3, 6, and 8 showed moderate α amylase inhibitory effect. This effect might be due to the presence of alkaloids, flavonoids, saponins, tannins and sterol, which was observed by preliminary phytochemical screening. Conclusion: This study showed that the herbal formulation of ethnobotanical plant extracts may be used to control hyperglycemia