Analgesic and Anti-inflammatory Properties of Argyreia Pilosa Wight & Arn

Background: Pain and inflammation are usually related to the pathophysiology of several medical conditions. The majority of antinociceptive and anti-inflammatory medications accessible within the merchandise found an outstretched purview of complications. The existing investigation was focused at identify the constituents and investigating the antinociceptive and anti-inflammatory activity of methanol extract of Argyreia pilosa whole plant. Methods: The dried plant of A. pilosa was extracted with methanol and partitioned between Petroleum ether, chloroform, ethyl acetate, and butanol. The organic layer was fractionated by various stationary phases and identified by using spectral analysis. The methanolic extract was assessed for its antinociceptive activity by utilizing hot plate, tailimmersion and acetic acid induced writhing test, while anti-inflammatory activity has been established by Carrageenan induced paw edema carried out in vivo. The test group acquired two different doses of the extract (200 mg/kg and 400 mg/kg) orally. The positive control group acquired diclofenac (5 mg/kg), tramadol (2 mg/kg) orally. The negative control group acquired vehicle (2% Tween 80, 10 ml/kg) orally. Additionally, preliminary phytochemical screening was performed. Results: Phytochemical investigation of the whole plant of Argyreia pilosa (Convolvulaceae) afforded four known Phytoconstituents identified as Stigmasterol (1), ß-Sitosterol (2), Rutin (3) and Quercetin (4). The structures of those phytoconstituents have been elucidated based on spectral information analysis. Oral administration of A. pilosa methanol extract (at all doses) considerably (p < 0.05) interdict pain sensitivity in the pain models. In the same manner, the extract exhibited anti-inflammatory activity in the inflammation models in the rat. Preliminary phytochemical screening showed the presence of flavonoids, alkaloids, tannins, phenols, steroids, fixed oils, fats, acid compounds, glycosides, amino acids, and proteins. Conclusion: The outcome attained from the current investigation signifies that the extract owned a considerable antinociceptive and anti-inflammatory activity may be attributed to the presence of isolated constituents, indicating the traditional relevance of the plant and recommended to determine the other active chemical constituents accountable for the antinociceptive and anti-inflammatory activities.