SELECTIVE AND NON-SELECTIVE ACTIVATED AND INHIBITORY AGENTS EFFECTS ON ADENYLYL CYCLASE IN THE KIDNEY OF THE RATS

Objective: To have in depth knowledge about the effects of nonselective and selective inhibitory and activated agents on adenylyl cyclase in rat kidney. Design: A variety of concentrations of pharmacological agents were prepared. They include nebularine, Ap3A, forskolin, Ap4A and caffeine. Furthermore, effects of these agents were noted in relation to rat kidney adenylyl activity. Methods: Tissue of rat kidney was used in the process of preparation of crude extract. Activity of adenylyl cyclase in connection with crude extract was observed. [2-H3] ATP was used as substrate which ultimately lead to the formation of cAMP. Pharmacological agents with their prepared concentrations were tested. Prominent activator of adenylyl cyclase, forskolin, was selected as a compound. Ap4A, caffeine, nebularine, and Ap3A were utilized for comparison purpose. Results: Adenylyl cyclase activity was at peak at 100 M forskolin as per concluded results. Nebularine inhibited activity of enzyme when agent concentration enhanced up to 50 M where the inhibition started to stable. No considerable effect on the enzyme activity in kidney tissue was observed when caffeine with 10 – 300 M on the of adenylyl cyclase activity was used. No effect on adenylyl cyclase activity was noted when Ap3A over the concentration range of 10 – 300 M was used. However, an inhibition effect on the enzyme activity was noted when Ap4A with the concentration 100 M was used. Conclusion: Role of cyclic nucleotides in metabolism control and cell-signaling is undeniably significant. It stimulates inhibitors and activities of cyclase to make some likely physiological impacts. Foreskin being initiator of adenylyl cyclase, Ap4A and nebularine are established to be latent inhibitors of cyclase, which signifies their importance in curing schizophrenia, mania, seizure etc. Key Words: Ap3A, Nucleotides, Caffeine, Forskolin, Ap4A, Nebularine. Adenylyl cyclase