VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF OMEPRAZOLE AND OFLOXACIN

A simple, Accurate, precise Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was developed for the simultaneous estimation of the omeprazole and Ofloxacin in Tablet dosage form. Chromatogram was run through ODS (150 X 4.6mm, 5µ). Mobile phase containing Buffer and Acetonitrile in the ratio of 45:55 was pumped through column at a flow rate of 0.8 ml/min. Buffer temperature was maintained at 30°C. Optimized wavelength for Omeprazole and Ofloxacin was 220nm. Retention time of Omeprazole and Ofloxacin were found to be 2.16 min and 3.39 min. %RSD of the Omeprazole and Ofloxacin were and found to be 0.62 and 0.74 respectively. %Recover was Obtained as 100.07% and 100.72% for Omeprazole and Ofloxacin. LOD, LOQ values were obtained from regression equations of Omeprazole and Ofloxacin were 0.27ppm, 0.37ppm and 0.83ppm, 1.13ppm respectively. Regression equation of Omeprazole is y = 11878x + 281.6, and of Ofloxacin is y = 14453x + 3910.2. Key Words: Omeprazole, Ofloxacin, RP-HPLC, LOD, LOQ