Effect of IN-OVO injection with Nano Iron -Particles on Physiological Responses and Performance of Broiler Chickens under Saini Conditions

A total of 600 fertile eggs, in a completely randomized design were used to investigate the effects of Iron nano-particles IN-OVO injection on productive performance, immune status and physiological responses in broiler chickens. The eggs were divided into 6 groups that assigned as: T1 (control; without injection), T2 (injected with 0.1 ml saline 9.0%; sham control), T3; (injected with 0.1 ml of 20 ppm Fe-NPs organic, T4 (injected with 0.1 ml of 20 ppm Fe-Nano inorganic), T5 (injected with 0.1 ml of 20 ppm Fe organic) and T6 (injected with 0.1 ml of 20 ppm Fe-inorganic). At 7th day of incubation, the corresponding doses were in- ovo injected in 0.1 ml solution into the air sac. The results showed that: Hatchability was highly significant (P< 0.01) in T1, 0.1 ml of 20 ppm Fe-NPs, 0.1 ml of 20-ppm Fe-NPs-Alimet chelate, 0.1 ml of 20 ppm Fe-Aliment chelate and 0.1 ml of 20-ppm Fe-Aliment chelate. The egg weight was higher (P< 0.01) in T2. There was an increase (P< 0.01) in chick weight in controls, other Fe-NPs organic or Fe-NPs- inorganic and Fe organic in comparison with other treatments. In addition, chick body weight to egg weight ratio in controls, Fe-Nano organic and FeNPs- inorganic was higher (P < 0.01) than in the other groups. T3 has shown the highest (P< 0.01) relative weight compared to the other treatments. Serum Fe content and liver function were (P< 0.01) higher in by using Fe-NPs, Fe-NPs alimet inorganic and Fe-organic than other treatments. The treatments of Fe-NPs- organic and Fe-Aliment chelate, chickens' blood hemoglobin increased significantly compared with the other treatments. These results suggest that Fe-NPs, Fe-NPs-Alimet chelate and Fe-Alimet chelate improved embryonic growth and development.