Isolation of stem cell populations from wharton’s jelly sections of umbilical cord and comparison analysis with cord blood stem cells

Wharton’s jelly (WJ) is a gelatinous tissue within the umbilical cord that contains myofibroblast like stromal cells. A unique cell population of WJ that has been suggested as displaying the stemness phenotype is the mesenchymal stromal cells (MSCs). Study designed to characterize the primitive stem cell content of cord blood and its wharton’s jelly with regard to neonatal parameters. Methods: Both cord blood and wharton’s jelly and their CD34+ and CD90+ expression was enumerated by flow-cytometry. Neonatal parameters like birth weight, gender, gestational age and mode of delivery were analyzed to correlate with CD34 and CD90 markers. Results: From a total of 20 deliveries, CD34 and CD90 expression pattern showed varied in both samples. Gender and the expression of these proteins (CD34 and CD90) doesn’g shown any significant. Wheras, correlation analysis of weight with CD34+ and CD90+ cell counts revealed that birth weight showed significant and it may have effect on both cell counts. Conclusion: Neonatal parameters expecially birth weight may have the affect on cell count of CD34 and CD90 in both CB and WJ. Findings provide a fast and efficient method for mesenchymal cell isolation from Wharton's jelly using the total length of the UC, which can be easily applied, along with UC blood, for UC banking.