INVESTIGATION ON THE SCALE-UP CRITERIA FOR FUNGAL FERMENTATION IN SHAKE FLASK

To perform scale-up of fermentation in a shake flask, which the operation efficiency is equivalent to the smallscale bioreactor, many experiments are required to investigate the desired operational conditions. It was needed considerable amounts of material and time. Therefore, in this study, during fermentation of Aspergillus oryzae using in shake flasks with different sizes the efficient hydrodynamic parameter for changing the scale by keeping the productivity has been obtained. Finally, results show the same shaking frequency is the most appropriate scaleup criteria that is resulted in a high enzyme activity and biomass by controlling the cell morphology parameters.