USING OF LOWLAND PEAT SUBSTRATE FOR CULTIVATION OF PICHIA ANOMALA IMB Y-5067 AND RHODOTORULA GRACILIS IMB Y-5075 YEAST STRAINS

The aim of the research was to study the accumulation of yeast lipids in the process of cultivation of strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 with the use of peat as growing substrate. The object of researches was strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 from “Collection of strains of microorganisms and plant lines for food and agricultural biotechnology” of SE “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”. As a raw material, lowland peat which was preliminarily processed with the help of cavitation or explosive autohydrolysis was used. The accumulation of lipids over the course of cultivation P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 on non-food substrate ‒ lowland peat was shown. The effect of pulp explosive autohydrolysis and pulp cavitation processing on biomass accumulation and lipids synthesis by strains P. аnomala IMB Y-5067 and R. gracilis IMB Y-5075 was researched. It was found that the maximum lipids accumulation by strains P. аnomala IMB Y-5067 (9.7 g/dm3) and R. gracilis IMB Y-5075 (8.9 g/dm3) was over the course of cavitation processing of peat pulp and additional application of salts and yeast extract into cultivation environment.