BIOCHEMICAL STUDIES ON AMELIORATIVE EFFECT OF PROBIOTIC ON INDUCED OBESITY IN RAT

Obesity is a significant cause of morbidity and mortality worldwide. Obesity was induced in our experiment by consumption of diet containing 15% palm oil and by adding of sucrose (20%) to the drinking water for a period of 16 weeks. Probiotics have several health benefits so we used PROBAC Plus (20 million mos /g.) to evaluate its ameliorative effect on biochemical changes associated with obesity. It composed of Lactobacillus Acidophilus, Lactobacillus Planterum, Lactobacillus Bervis, Bifidobacteriais and Saccharomyces Cerevisiae. 80 male albino rats were divided into control group (Cr), High fat diet &sucrose group (HFDS), probiotic group (Pro.), High fat diet & sucrose + probiotic group (HFDS+ Pro.) and High fat diet& sucrose then probiotic group (HFDS then Pro.). Consumption of palm oil and sucrose for 16 weeks leads to a significant increase in body weight and abdominal fat mass compared to normal rats. In addition, there was a significant increase in the level of serum triacylglycerol, total cholesterol, LDL-C, liver enzymes and leptin hormone. Malondialdehyde (MDA) and TNF-α concentration of liver homogenate were also significantly increased. It was also observed a significant reduction in serum level of HDL-C. The concentration of SOD and reduced glutathione (GSH) in liver homogenate were reduced. Use of probiotic as proflactive or as treatment return serum lipid profile, liver function enzymes, antioxidant parameters, TNF-α and leptin hormone towards its normal value. These results confirm the beneficial role of probiotic for treatment of obesity.