An Interaction Study of Metal Ions Complexation with Cephradine on Its Antibacterial Activity

This research work comprises of interaction studies of cephradine with essential metals and investigation of antimicrobial activity of cephradine. Cephradine is included among the cephalosporin drug class which is active against a wide range of gram positive and negative bacteria. Cephradine has been interacted with Zn, Fe metal salts in vitro. All the reaction conditions were simulated to natural environments. The antimicrobial activity of the drug and the complexes also were determined. The studies were carried out in buffer at PH 7.4 in variable ratios of drug and metal both at room temperature. It has been observed that almost major proportion of drug is complexed with metals. There is a effect of PH on drug metal complexation. It has been observed that cephradine interacts with metals a PH 7.4. The stability constant of these complexes were determined in order to evaluate their possible in vivo implications. In order to investigate the number of metal ions involved in the complexation with cephradine complexes were elucidated by exploiting various spectrophotometric methods. The ultraviolet studies of these complexes were carried out and compared. The test samples were tested against Staphylococcus aureus. The standard cephradine disk also tested against Staphylococcus aureus. We observed that the antimicrobial activity of cephradine decreases when it forms complexes with ZnSO₄.7H₂O and FeSO₄.7H₂O by antimicrobial studies.