Evaluation of Antioxidant Potential of Zingerone against High Fructose Diet Induced Non-Alcoholic Steatohepatitis in Rat Model

Zingerone was proven to possess many pharmacological functions including hepatoprotective, antiparasitic, antiflarial, antimicrobial, antidiabetic and radio protective properties. The aim for this study was to evaluate the antioxidant potential of zingerone against high fructose diet induced NASH. A total of 24 rats were used in this in vivo study. The rats were divided into four groups (n=6) and treated as follows for 8 weeks: Group 1 (CON): Rats received normal standard pellet diet with water, Group 2 (ZIN): Rats received normal pellet diet and zingerone (100 mg/kg body wt /day), Group 3 (HFD): Rats received high fructose diet (40% of fructose and rodent chow with drinking water (25% fructose). Group 4 (HFD+ZIN): Rats received high fructose diet and zingerone (100 mg/kg body wt /day). DPPH radical scavenging activity and total phenolic content assay were done to determine antioxidant potential of zingerone as for the preventive effect of NASH. The results indicated that the zingerone (100 mg/kg body weight) treated group showed the highest scavenging activity in both normal diet rats (ZIN): and high fructose fed rats (HFD+ZIN) which was found to be 129%, and 125% respectively. The total phenolic content and gallic acid concentration was found to be higher in the gingereone treated group (ZIN) indicating the antioxidant potential of gingerone. Hence zingerone may have antioxidant potential against high fructose diet induced non-alcoholic steatohepatitis in rats