Implication of Biofield Energy Healing Based Vitamin D3 on Osteoblastic Differentiation

Bone is one of the dynamic organ of the endoskeleton, which play a vital role in maintain the structural integrity, blood production, coagulation, mineral reservoirs, and body immunity. The present study investigates the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in MG-63 cell line. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts, and each sample received the Consciousness Energy Healing Treatment by Su-Mei Chen Liu and labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization as bone biomarkers were studied. The cell viability using MTT assay showed more than 84% in test items groups were found to be safe. ALP level was significantly increased by 217% (100 µg/mL), 272.8% (10 µg/mL), and 233.9% (100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively. Collagen content was significantly increased by 121.6%, 134.3%, and 86% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 100 µg/mL as compared with the untreated group. Additionally, the percent of bone mineralization was significantly increased by 213.5% (at 10 µg/mL), 109.5% (at 100 µg/mL), and 283.7% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D3 and DMEM would play an important role to improve the bone mineralization and calcium absorption. The bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Further, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget's disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss, and fractures.