Highlights of CRISPR-Cas9 Genome Editing

The advantage of CRISPR genome editing is not limited to only its cost, but it is easy to use in any lab with molecular biology expertise. On the other hand, CRISPR does not need to protein engineering for targeting each gene, while TALE and ZF nucleases are needed. CRISPR-Cas9 genome editing system needs only a DNA construct to encode the target specific gRNA and Cas9 in one expression vector. Furthermore, CRISPR-Cas9 can edit multiple genome with homolog sequencing simultaneously, resulting in increasing the efficiency