Micropropagation of Atropa acuminata Royle from in vitro petiole explant

During the present study, in vitro petiole explants of Atropa acuminata were subjected to in vitro studies so as to develop efficient micropropagation protocols for its regeneration. In vitro petiole explant produced maximum amount of callus on MS medium supplemented with BAP (3mg/l) in 80% cultures within 20 days. Shoot regeneration was obtained after sub-culturing the callus on MS medium supplemented with BAP with mean shoot number of 8.6-±4.22cm and a mean shoot length of 2.3-±0.20 cm in 100% cultures within 13 days. Root regeneration was obtained on MS medium augmented with IBA (0.5mg/l) with a mean number of roots 6.0-±2.50cm and mean root length of 1.3-±0.11cm with 60% response within 32 days. Farhana Maqbool | Seema Singh | Mahroofa Jan"Micropropagation of Atropa acuminata Royle from in vitro petiole explant" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd8376.pdf http://www.ijtsrd.com/biological-science/botany/8376/micropropagation-of-atropa-acuminata-royle-from-in-vitro-petiole-explant/farhana-maqbool