Taguchi Method Approach on Preconcentration and Speciation of Trace Amounts of Inorganic and Organic Selenium in Mushroom Samples with Cloud Point Extraction and Uv-Vis Spectrophotomety

A micelle-mediate extraction method was developed for the speciation of selenium in mushroom samples with cloud point extraction (CPE) as a separation and preconcentration method, and determination by UV-visible spectrophotometry. The CPE is based on the complex of Se(IV) with 2,3-diaminonaphthalene (DAN) and then entrapped in non-ionic surfactant, Triton X-114.Taguchi method was used to optimize the main factors affecting CPE efficiency, such as pH of sample solution, concentration of Triton X-114 and DAN, equilibration temperature and time. Under the optimal conditions, the detection limit of this method for Se(IV) is 9 ng mL^(-1), and relative standard deviation is 3.8% at determination of 50 ng mL^(-1) Se(IV). The proposed method has been applied for determination of trace amounts of inorganic and organic selenium in mushroom samples with satisfactory results.