PECULIARITIES OF ANTIBIOTIC BATUMIN ACTION ON BIOFILM FORMATION BY Staphylococcus aureus AND Pseudomonas batumici

The aim of the work was the research of antibiotic batumin action on the biofilm formed by staphylococci and on the process of the biofilm formation by strain-producer Pseudomonas batumici. The minimal inhibiting concentration (MIC) of batumin was studied according to The Clinical and Laboratory Standards Institute standards — CLSI. Biofilm formation was studied using photometric O'Toole method with flatbed photometer at wavelength 540 nm. The batumin penetrated into biofilms of staphylococci and reduced the biomass of the formed biofilm of Staphylococcus aureus and S. epidermidis at the antibiotic concentration of 0.5 μg/ml, which was only twice higher than MIC of batumin for planktonic cells. The degree of biofilm changes in the presence of batumin differed for different staphylococcus strains. However, these differences did not depend on strains sensitivity or their resistance to the antibiotic. The ability of batumin strain-producer to form the biofilm and the ability of antibiotic to stimulate this process in concentrations of 1 and 10 μg/ml was established for the first time. In addition, it was found that in the Pseudomonas batumici B-321 planktonic culture in the presence of 10 μg/ml of batumin, the increase in length of producer cells on average by 15% was noted. The obtained data shed the light on batumin antimicrobic action on the staphylococci biofilm. It can be argued that is a perspective for treatment of staphylococcal infections. It enabled to consider batumin as a promising drug for the staphylococcal infections treatment.