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IN VITRO INTRODUCTION AND MICROPROPAGATION OF TWO HONEYSUCKLE SPECIES

Journal: Science and world (Vol.2, No. 49)

Publication Date:

Authors : ;

Page : 16-18

Keywords : in vitro; micropropagation; explant; agar nutriculture medium;

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Abstract

The results of optimization of nutrient solution's composition f or in vitro introduction and micro- propagation of two Kazakhstan honeysuckle species Lonicera altaica and L. iliensis are given in this article. For in vitro introduction of L. altaica the most suitable was the Murashige and Skoog medium with 0,25 mg/l of b enzylamino- purine; for micropropagation – medium with the lowered concentration of macronutrients and minor - nutrient element (¾ MSO) with 2 mg/l of benzylaminopurine. For in vitro introduction of L. iliensis – the MSO with 0,25 - 0,5 mg/l of ben- zylaminopurine ; for micropropagation – MSO with 0,5 mg/l of benzylaminopurine and the doubled concentration of ferric chelate.

Last modified: 2018-08-09 21:09:33