Evaluation of the Experimental Model Quality of the Chronic Sinusitis of the Maxillary Sinus Based on Morphometry Data

The purpose of this work is to investigate the dynamics of morphometric changes in the process of modeling chronic sinusitis of the maxillary sinus and to determine its compliance with this diagnosis, that will be a quality assessment of the proposed experimental model. Materials and Methods. The study was performed on 12 male rabbits, which were established models of chronic sinusitis of the maxillary sinus. Time morphometric studies were as follows: 14 days, 1 month, 2 months, 3 months determined in accordance with the periods of reparative regeneration of bone tissue, and also in accordance with the changes occurring in the mucosa lining the maxillary sinus. Morphometry was performed by the standard technique of serial sections by counting inflammatory cell infiltrate (segmented leukocytes, eosinophilic granulocytes, lymphocytes, plasma cells, macrophages, giant multinucleated cells) and the number of elements in the vasculature and fibroblasts, reflecting the reparative regeneration processes. Then, all the obtained data were processed using the methods of medical statistics and the Statistics 10.0 program package. To assess the change in cell populations at different stages of observation, the Kraskel-Wallis criterion (H) was used. Counting was performed in 96 fields of vision. Results and Discussion. The results allow concluding that the offered method of creation of experimental model of chronic sinusitis of the maxillary sinus corresponded to the clinical situation when the violation of the technology of endodontic treatment filling material entered into the lumen of the sinus or under the mucous membrane lining it. This confirms the adequacy and quality of the developed experimental model, determining its suitability for studies of the effectiveness of the improvement of known and development of new treatments for chronic sinusitis of the maxillary sinus. Conclusions. Everything listed above both clinically, and morphologically corresponds to signs of chronic inflammatory process, and, therefore, confirms adequacy and quality of the developed experimental model, defining its suitability for an efficiency research at improvement known and development of new methods of treatment of a chronic sinusitis of a maxillary sinus.