Extraction and isolation of anti-hypertensive peptide by alkalase from spirulina platensis

Spirulina has attracted a great attention as it contains many nutrients, such as protein, vitamins and minerals. Spirulina contains peptides that have therapeutic and beneficial effects on the human body. Some medicinal properties of the biological peptides of Spirulina platensis include antioxidants, antifungal, antimicrobial, anti-diabetes and anticoagulant activity. This study provides an overview of the biological peptides derived from Spirulina and some biological activities with health benefits. In this study peptide Ile-Gln-Pro from spirulina platalsis was isolated using an alkalase enzyme and investigate its inhibitory effect on the angiotensin I-converting enzyme (ACE). This peptide was purified by gel filtration chromatography and Reverse-phase high-performance liquid chromatography (RP-HPLC) Enzymatic kinetic studies showed a non-competitive inhibitory activity of this peptide, and the Ki value was 5.8 μm.