Effect of Coconut Water with Egg Yolk Combination and Storage Length on Spermatozoa Abnormality and Membrane Integrity of Bali Bull

This study aimed to investigate the effect of coconut water with egg yolk combination in the extender and storage length on liquid spermatozoa abnormality and membrane integrity of Bali bull. Method used in this study was experiment in a completely randomized factorial design with 2 factors. The first factor was the level of coconut water and egg yolk which consisted of 5 treatments, including P0 (control) = Tris amino methane, P1 = 85% coconut water + 15% egg yolk, P2 = 80% coconut water + 20% egg yolk, P3 = 75% coconut water + 25% egg yolk, P4 = 70% coconut water + 30% egg yolk. The second factor was the length of storage at low temperature which consisted of 3 treatments, including 1 day, 2 days and 3 days. All treatments were replicated 10 times. Variables observed were spermatozoa abnormality and membrane integrity. Results showed that the abnormality value of spermatozoa in P0 after 3 days of storage was 12.54%. This value was lower (P<0.05) than in P1, P2, P3 and P4 groups with 16.52%, 15.62%, 14.66%, and 13.65% of abnormality value, respectively. Membrane integrity value of spermatozoa which was diluted in P0 treatment after 3 days of storage was 66.23%, which was significantly higher (P<0.05) compared to those diluted in P1 56.93%, P2 59.23%, and P3 61.73%, but not significantly different compared to P4 64.51%. The conclusion of this study is that the use of the extender with the ratio of 70% coconut water and 30% egg yolk could maintain the spermatozoa abnormality and membrane integrity of Bali bull.