Effect of He-Ne Laser (632.8 Nm) in Fluorescence Efficiency of Solvents

In this work, the laser of He-Ne absorbed by beet dyes makes effects of the dyes, which studied as PH effect. The dyes solution was separated by using tow technique; the HPLC-absorption and HPLC fluorescence techniques to get information about the number of the components present in the dye solution and both techniques give the same result indicating the presence of four components. The dye solutions were pumped by He-Ne laser (632.8 nm)and the fluorescence efficiency was displayed by (usb 2000 spectrophotometer detector).the proportional relation between absorbance and fluorescence was investigated in this study.