Synthesis, Characterization and Imaging of Fluorescine Isothiocyanate Conjugated Magnetite Nanoparticles in MCF 7 Breast Cancer Cell Lines

In this work we describe fabrication, characterization and possible application of FITC (fluorescine isothiocyanate) conjugated magnetite nanoparticles (MNPs) for biomedical applications such as imaging of cancer cells. The MNPs possessed octahedral-like geometry with almost completely dispersed distribution with high saturation magnetization. The final FMNPs (fluorescine isothiocyanate magnetite nanoparticles) absorption band showed 30 nm red shift towards longer wavelength compared to bare MNP and the laser-induced fluorescence was observed at 518 nm. The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) results showed that FITC conjugation diminishes the toxicity MNPs mainly due to the reduction of surface charge. Fluorescence microscopy confirmed the uptake and distribution of FMNPs in MCF 7 (Michigan Cancer Foundation-7) breast cancer cells, which suggests that it can be utilized for applications such as a magnetic fluorescent probe for bioassay.