A New Spectrophotometric Determination of Endosulfan and Its Application

"ABSTRACT A new spectrophotometric method has been developed based on the modification of fujiwara reaction for the determination of endosulfan in sub ppm level. Endosulfan on its alkaline hydrolysis gives chloroform, which can be reacted with pyridine to produce pink colour. In the present method the pink colour obtained in the above reaction is discharged with a few drops of acetic acid followed by the addition of p-aminoazobenzene reagent which gives an orange coloured dye. The absorbance maxima of orange coloured dye formed is measured at 490 nm. Beer’s law is obeyed over the concentration range of 1- 36 ?g in a final solution volume of 25ml. The molar absorptivity and Sandell’s sensitivity were found to be 6.71×105 Lmol-1 cm-1 and 0.22×10-3?g cm-1 respectively. The standard deviation and relative standard deviation for the absorbance value were found to be 0.0148 and 4.05% respectively. This method is free from interference of other organochlorines pesticides and can be successfully applied for the determination of endosulfan in various environmental samples. "