ISOLATION, CULTURE AND MORPHOLOGICAL CHARACTERIZATION OF MICROCYSTIS SP TOXIC STRAIN FROM THE TACUARY RESERVOIR
Journal: International Journal of Advanced Research (Vol.6, No. 8)Publication Date: 2018-08-08
Authors : Victoria Lopez Pereira; Estelvina Rodr guez Portillo.;
Page : 387-393
Keywords : Microcystis isolation culture cell density growth rate.;
Abstract
It was studied the growth of the cyanobacterium Microcystis sp. isolated from algal blooms of the Tacuary reservoir. The cultivation was performed in triplicate BG11 medium, average temperature of 25 ? 3 ? C, 0.8086 ?mol?s⁻??m⁻?, with a photoperiod of 14:10, pH of 7.10 and daily stirring. Isolation was performed by means of the pipette method and sequential dilutions. The isolated culture revealed a maximum growth rate of 0.357 days -1 and minimum of 0.12 day -1 on average doubling time of 4.124 days. During the analysis, the strain increased its density through the acceleration and exponential phases. It reflects in a short time that the strain is conserved with a high rate of cell evolution under controlled conditions; consequently, it contributes to the comprehension of the phenomenon behavior for its management or environmental control.
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