THE ROLE OF GUT INDIGENOUS MICROBIOTA IN THE DEVELOPMENT OF OXIDATIVE STRESS IN WOMEN WITH RECURRENT PYELONEPHRITIS

The aim of our study was to investigate the oxidative stress intensity depending on the content of Lactobacillus spp. in the colon of patients with recurrent pyelonephritis. Material and methods. The observational study involved 60 women with chronic kidney disease (CKD) stage I-II: uncomplicated pyelonephritis, aged 39.5 ± 3.2 years. According to the quantitative content ofLactobacillus spp. in the patients' intestine, the women were allocated into two groups: the first group of the patients (n = 34) had a deficit ofLactobacillus spp. in the intestine, and the second one (n = 26) had a normal content of Lactobacillus spp. Along with the standard diagnostic methods, we defined the content of malondialdehyde levels in the serum (MDAs) and erythrocytes (MDAe) spectrophotometrically as an indicator of lipid peroxidation. Such parameters as the concentration of ceruloplasmin (CP), transferrin (TR) and sulfhydryl groups (SH-groups) in the blood and total peroxidase activity (TPA) in erythrocyte were studied as the indicators of antioxidant system. In addition, we calculated of oxidative stress index (OSI). Results. The analysis of lipid peroxidation defined a significant increase of MDA level in the serum (p = 0.008) and urine (p = 0.003) ofpatients with deficit of intestine lactobacillus spp. Moreover, in the patients ofGroup II we observed the high level of serum OSI (p = 0.03). By contrast, the TPA level was significantly reduced (p = 0.02). Conclusions. The results of our work confirm the experimental studies data, which demonstrate the leading role of gut indigenous microbiota in the development of oxidative stress.