Azadirachta Indica A. Juss. Leaf Extract Mediated Synthesis of Silver Nanoparticles and their Antibacterial Efficacy Against Selected Human Pathogens
Journal: International Journal of Trend in Scientific Research and Development (Vol.2, No. 5)Publication Date: 2018-09-26
Authors : T. Leon Stephan Raj J. Vijayakumari M. Menaga;
Page : 1199-1204
Keywords : Biotechnology Azadirachta indica; FT-IR; Antibacterial activity; Green synthesis and Silver anaoparticles;
Abstract
The present study deals with the biological synthesis of silver nanoparticles (AgNPs) from the leaves of Azadirachta indicia and their efficacy against human pathogens. Azadirachta indicia has the potent medicinal properties and used in different aspects of traditional medicines. The presence of silver nanoparticles was confirmed by the formation of brown color of the reaction mixture. The green synthesized silver nanoparticles were characterized by Fourier transmission infrared spectroscopy (FT-IR) analysis and X-ray diffraction (XRD). FT-IR revealed that the functional groups were observed and capped with plant secondary metabolites. X-ray diffraction was used to confirm the crystalline nature of the particles. Antibacterial activity of silver nanoparticles was performed by agar disc diffusion method against Staphylococcus aureus, E. coli, Klebsiella pneumoniae, Enterococcus faecalis and Pseudomonas aeruginosa. The highest zone of inhiitionof silver nanoparticles synthesized by Azadirachta indica extracts was found against Klebsiella pneumoniae (17 mm). The silver nanoparticle synthesized in this process has the efficient antibacterial activity against pathogenic bacteria. It can be used in the field of medicine, due to their high antibacterial activity. T. Leon Stephan Raj | J. Vijayakumari | M. Menaga"Azadirachta Indica A. Juss. Leaf Extract Mediated Synthesis of Silver Nanoparticles and their Antibacterial Efficacy Against Selected Human Pathogens" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd16986.pdf http://www.ijtsrd.com/biological-science/biotechnology/16986/azadirachta-indica-a-juss-leaf-extract-mediated-synthesis-of-silver-nanoparticles-and-their-antibacterial-efficacy-against-selected-human-pathogens/t-leon-stephan-raj
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