Effect of Î’-Citronellol on Hydrolytic Enzyme Secretion, Ergo sterol Biosynthesis and Bio film Formation in C. Albicans

Antifungal potential of ß-citronellol was studied on C. albicans, a commensal that causes both superficial and systemic infection in immunocompromised humans. This non-toxic natural compound displayed a minimum inhibitory concentration (MIC) of 200 µgmL and was fungicidal at 350 µgmL. On YPD agar, it formed inhibition zones (ZOI) of 16.2 mm, 18.15 mm and 19.25 mm respectively, at MIC, 2MIC and 3MIC while fluconazole (FLC) formed ZOI of 20.43 mm at its MIC (disc diffusion assay). No growth was observed above 2MIC on solid media (spot assay) and = MIC in liquid YPD media. Hydrolytic enzyme secretion decreased in the presence of ß-citronellol and was concentration dependent. At MIC, the reduction in phospholipase activity (54.08 %) was greater than the reduction in proteinase activity (40.6 %). There was concentration dependent decrease in total ergo sterol content by 19 %, 40 %, 91 % and 100 %, respectively at MIC8, MIC4, MIC2 and MIC values of ß-citronellol. FLC at MIC showed an inhibition of only 64%. Biofilm formation reduced by 71.13% at MIC. ß-citronellol, hence has immense antifungal potential and significantly inhibits growth, ergosterol levels, hydrolytic enzyme secretion, adhesion and biofilm formation in C. albicans at MIC and sub-MIC values. Sumit Kumar Rastogi | Yamini Sharma | Nikhat Manzoor"Effect of Î'-Citronellol on Hydrolytic Enzyme Secretion, Ergo sterol Biosynthesis and Bio film Formation in C. Albicans" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd18305.pdf http://www.ijtsrd.com/biological-science/microbiology/18305/effect-of-β-citronellol-on-hydrolytic-enzyme-secretion-ergo-sterol-biosynthesis-and-bio-film-formation-in-c-albicans/sumit-kumar-rastogi