Comparative study of protease activity of psychotrophic and mesophilic bacteria

Six bacterial colonies were isolated from solid agar medium containing skimmed milk from Ice sample from juice corner (Lucknow). Among these three isolates were found to be capable of producing protease alkaline pH and at different temperature ranging from 20-45 ° C. On the basis of larger clear zone formation at low temperature, i.e. 20 ° C, these three isolates was selected as potent protease producer strain, which are gram positive designated as BBAU/P-1, BBAU/P-2, BBAU/P-3. Eight bacterial colonies were isolated from skimmed milk agar from Soil sample of Garden. Among these four isolates were found to be capable of producing protease at different temperature, On the basis of larger clear zone at high temperature, i.e. 45 ° C. These four isolates designated as BBAU/P-4, BBAU/P-5, BBAU/P-6, and BBAU/P-7. Out of these seven protease producing bacteria, BBAU/P-5 produced high amount of protein as assayed by the cup-plate method based on zone hydrolysis around the well in solid media.Thus, results suggestmesophilic bacteria has more potential to produce protease as compare to psychotrophic bacteria.