Bioactive potential from Marine sponge Callyspongia diffusa associated Pseudomonus fluorescens BCPBMS-1 and Penicillum citrinum

The exploration for marine sponge associated novel microbes, producing rich and highly potential therapeutic metabolites, could diversify the scopes in life sciences. Since this has remained mostly untouched, the research was carried out to explore the bioactive potential of a marine sponge, Callyspongia diffusa associated microbes. The strains selected from the C. diffusa were Pseudomonas fluorescens and Penicillum citrinum and their cell free extracts were tested for hemolytic activity on sheep blood agar media and antioxidant activity was assessed with lyophilized cell free extracts. Anticancer activity was performed by cytotoxicity assay against HEP-2 cell lines. Cell free extracts of both P. fluorescens and P. citrinum demonstrated α-hemolysis on sheep blood agar. The lyophilized culture filtrate of P. fluorescens BCPBMS-1 and P. citrinum exhibited concentration dependent antioxidant activity revealing a positive linear relationship and ca. 85% and 74% antioxidant activities were obtained respectively with 1.0 mg/ ml of each of the sample. In case of cytotoxicity assay, P. citrinum demonstrated maximum viability of 96.61% at 1.95 µg/ ml of lyophilized culture filtrate and minimum viability of 20.33% at 1000 µg/ ml. The study proved that both P. fluorescens BCPBMS-1 and P. citrinum strains produce bioactive metabolites with hemolytic activity and antioxidant activity whereas P. citrinum could be a valuable resource for anticancer metabolites.