Evaluating the Correlation of Different Culture Media for the Identification and Isolation of Common Gram Positive Uropathogens and Yeast Causing UTI

Background: Urinary tract infection (UTI) is a common problem diagnosed and treated in urgent care medicine practice. Urinary tract infection (UTI) is a collective term that describes any infection involving any part of the urinary tract, namely the kidneys, ureters, bladder and urethra. Clinicians need to be aware of the advantages and limitations of diagnostic tests. Urine cultures occupy most of the workload of routine microbiology laboratories in developing country . Accurate and rapid identification of pathogens is important for a clinical microbiology laboratory. Materials and Methods: Mid-stream urine and catheterized samples were collected. Cultures were plated on blood agar, MacConkey agar and cysteine lactose electrolyte deficient media and incubated overnight at 35°C-37°C in ambient air. Colonies on the MacConkey agar, CLED agar and blood agar were also identified. The final identification of the isolates was done using standard identification protocol. Results: Out of 500 urine samples processed, 199 showed pure growth .Out of 199 pure growths, 56 were gram positive cocci and 17 were yeast. Out of all gram positive cocci showed growth but most of the gram-positive cocci and yeast were unable to grow on Mac-Conkey agar and blood agar but grew successfully on CLED agar. Conclusions: So, in resource constrain laboratories, CLED agar can be used as media of choice for isolation of common uropathogens , cost effective and decreases work load of the laboratories.