Biosynthesis of Silver Nanoparticles by Chlorodesmis Hildebrandtii A. Gepp & E. Gepp Including its Agricultural and Biomedical Implications

The aim of this work was to investigate the biosynthesis of Silver (Ag) Nanoparticles from aqueous extract of green seaweed Chlorodesmis hildebrandtii and its characterization. The synthesized Ag-Nanoparticles were characterized using UV-Visible Spectrophotometer, Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering, Scanning Electron Microscopy and X-Ray Diffraction analysis. The biosynthesized Ag-Nanoparticles had been analysed for its inhibitory activity against some human pathogenic bacteria. The biosynthesized Ag-Nanoparticles had been tested for its potentiality to seed germination to confirm its Phyto-friendly nature and for its future use as bio-nano-fertilizer. The antibacterial activity was tested with agar disc diffusion method and the seed germination was also done according to standard protocol. The inhibitory activity of biosynthesized Silver Nanoparticles was the highest against Klebsiella pneumoniae (1.5±0.5cm), followed by Escherichia coli (0.63±0.05cm) and Proteus mirabilis (0.23±0.05cm) than the zone of inhibition of antibiotic Chloramphenicol (5mg/ml). The biosynthesized Ag-Nanoparticles had accelerating potentiality to seeds germination. The seed germination percentage was more in case of seeds treated with biosynthesized AgNanoparticles. The seed germination was 100 percent for both seeds of Abelmoschus esculentus and Raphanus sativus var. longinnatus. But seed germination percentage was less in case of seeds treated with normal water (60%), seaweed extract (60%) and seaweed liquid fertilizer (40%).