In vitro Isolation and Characterization of Mesenchymal Stem Cells (MSCs) from Lipoaspirate: a new Preclinical Protocol

Mesenchymal Stem Stem Cells (MSCs) could provide alternative therapeutic solutions for a number of bone and cartilage tissue diseases, playing an important role in regenerative medicine. Several sources of stem cells are currently available. In recent years, researchers attention was focused on the isolation of Adipose Stem Cells (ASCs) because they are easily obtainable in large quantity trough a minimally invasive procedure. Furthermore, Adipose Derived Stem Cells (ADSCs) have multiple properties, including self-renewal, differentiation potential, secretion of active factors that might influence surrounding cells and immune modulatory effects useful in the treatment of several degenerative diseases. The aim of this study was to present a new “in vitro” ADSCs isolation and characterization protocol using a mechanical method in comparison to the enzymatic digestion that has represented the gold standard procedure to isolate adult mesenchymal stem cells.