An Alternative of Phosphopeptide Identification using Inclusion List-Driven Targeted Liquid-Tandem Mass Spectrometry for Label-Free Quantitative Tyrosine Phosphoproteomics: Application of Src Inhibitor-Related Signaling Pathway Analyses in Lung Cancer

Tyrosine phosphorylation plays a critical role in cell biological functions, particularly in signal transduction. Mass spectrometry (MS)-based proteomic analysis of tyrosine-phosphorylated (pTyr) proteins remains challenging due to their relatively low abundance. The methods of phosphopeptide enrichment are developed prosperously such as IMmuno Affinity Chromatography (IMAC) and Metal Oxide Affinity Chromatography (MOAC). However, the complexity of experimental design for sample separation induces the unnecessary variations