Hey1 gene expression patterns during the development of branchial arches and facial prominences
Journal: REVISTA MVZ CÓRDOBA (Vol.23, No. 3)Publication Date: 2018-09-05
Authors : Belfran Carbonell M; Francy Bayona R; Zayra Garavito-Aguilar; Carolina Parada B; Humberto Arboleda G; Clementina Infante-Contreras;
Page : 6813-6825
Keywords : Chicken embryo; craniofacial development; facial ectodermal; frontonasal prominence; mandibular prominences; notch signaling;
Abstract
Objective. The present study aimed to describe in detail the expression patterns of the gene Hey1, an effector of the Notch pathway, during the development of branchial arches and facial prominences. Materials and methods. Fertilized chicken (Gallus gallus) eggs obtained from a local egg farm were incubated at 37.5 -38.5ºC with 70% relative humidity until the embryos reached Hamilton-Hamburger stages HH14 through HH25. Digoxigenin-UTP labeled probes Hey1 were generated from linearized plasmids with either T3 polimerase for in vitro transcription. Whole-mount in situ hybridization was then performed. At least 3 replicates (n=3) were obtained for each stage. To confirm the results observed in whole embryos, sagittal and coronal cryosectioning was performed using a thickness of 10 μm. Results. During developmental stages HH14 and HH18, Hey1 gene expression was localized to the endoderm of branchial pouches. Hey1 gene expression was also observed in the epithelium that covers the maxillary and mandibular prominences during developmental stages HH19 and HH21, as well as in the nasal epithelium between HH19 and HH25. Transcripts were also detected in the epithelium that covers the frontonasal prominence during stage HH21.Conclusions. These expression patterns suggest the participation of this component of the Notch signaling pathway in craniofacial morphogenesis, possibly establishing pharyngeal segmentation patterns during early stages and/or regulating cell proliferation and differentiation during the late stages of facial development.
Other Latest Articles
- Acetylcholinesterase activity and total antioxidant levels in dogs with mammary tumors before and after surgical removal
- In vitro production of gas methane by tropical grasses
- Gene expression of growth factor BMP15, GDF9, FGF2 and their receptors in bovine follicular cells
- Zoonotic emergence of coronavirus: a potential public risk for Latin America
- Educational actions for periodontal health in pregnant women
Last modified: 2018-10-09 23:40:12