Determination of Ascorbic Acid Levels in Hibiscus Sabdariffa (Zobo Rod) and Other Tropical Leaves

"ABSTRACT: Ascorbic acid level in zobo rod a multidimensional tropical leaf and other leaf samples were determined by titrimetric method using ammonium cerium (IV) sulphate and 2,6-dichlorophenolindophenol. It was discovered that leaves like zobo rod, orange, guava, lime, lemon, pawpaw and Icheku have high ascorbic acid content of 44.44mg/100g, 57.31mg/100g,40.80mgl/100g,41.35mg/100g, 41.35mg/100g,36.75mg/100g and 30.43mg/100g respectively while leaf of avocado pea and okra have low ascorbic acid level of 15.20mg/100g and 20.70mg/100g respectively. Results obtained using ammonium cerium (IV) sulphate showed higher level of vitamin C compared to that of 2,6-dichlorophenolindophenol though without large differences. Chemometrically the results obtained from the methods were compared and at 95% level of confidence was found to be significant. Key words: Hibiscus sabdariffa, chemometrics, Ammonium cerium(IV) sulphate, 2,6-dichoropheneindophenol.,titrimetry "