BIOCHEMICAL ANALYSIS AND PEPTIDE MODELING OF LYSOZYME IN INDIAN FENNEROPENAEUS INDICUS SHRIMP SPECIES

FAO defines aquaculture as "the farming of aquatic organisms, including fish, mollusks, crustaceans, and aquatic plants". Fanning here implies some form of intervention in the rearing process to enhance production, such as regular stocking, feeding and protection front predators. Aquaculture is the only alternative to mitigate the threats to the world's fisheries by taking the pressure off from wild fish stocks while supporting livelihoods and food production. Aquaculture production has increased tremendously over the past few decades and today accounts for almost a half of global fish production by weight, while production from wild fisheries has largely slowed or stagnated. The enzyme lysozyme is extracted from Fenneropenaeus indicus and it is estimated by using Lowry?s method. Lysozyme is biochemically analysed with SDS-PAGE and HPLC. The activity of the enzyme is assayed and characterized by determining the optimum pH and temperature. The effect of activator and inhibitor on lysozyme activity is obtained.The final results of our project clearly explain that the identified motif peptide sequence and the 3D peptide structure are potential candidates for drug docking studies and also act as novel molecular markers useful for pharmacoinformitics and clinical endocrinology studies.