Evaluation of Extraction Methods of DNA from Dry Collection Material of Urophora cuspidata and Urophora macrura (Diptera: Tephritidae)

Different DNA extraction protocols are evaluated for DNA isolation from various samples. In our study we compared three DNA extraction methods; a Chelex resin (C100), Qiagen DNA extraction kit and Cethyl Trimethyl Ammonium Bromide (CTAB) protocols obtained from dry collected materials of Urophora cuspidata and Urophora macrura (Diptera: Tephritidae) samples. Although, the hihgest yield of DNA was obtained from C100 method, the purest DNA was obtained with Qiagen protocol. Using RAPD-PCR, we demonstrate the efficacy of Qiagen protocol on these samples collected up to 6 years ago.