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Optimization of Xylanase Production from Fermentation of Water Hyacinth (Eichhornia crassipes) using Trichoderma species

Journal: Journal of Biotechnology Research (Vol.3, No. 3)

Publication Date:

Authors : ; ; ; ;

Page : 15-24

Keywords : : Xylanase; Extracellular enzymes; Microorganism; Environment.;

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Abstract

Department of Microbiology, Ahmadu Bello University Zaria Nigeria. Background: In the present study, optimized cultural conditions for enhanced production of xylanase from local soil isolate of Trichoderma species, using water hyacinth as a substrate in submerged culture fermentation is presented. Method: The Megazyme assay method was used for endo 1, 4-β-xylanase using Azo-xylan (Birchwood). Results: A continuous increase in xylanase production was observed with increasing level of substrate concentration in the medium and highest production was obtained with water hyacinth at 6% w/v level. Maximum xylanase production was achieved with a pH 5.0, incubation temperature of 30ºC and agitation rate of 150 rpm. The highest production was achieved on day five of fermentation at optimum parameters under study. Result: The study showed that production of xylanase can be cost effective using water hyacinth and can be implored on large scale for industrial applications.

Last modified: 2018-11-03 16:24:58