Some Kinetic Properties and Inhibition of Glutathione S-Transferase from a Hybridized Wheat (Triticum aestivum L.)
Journal: Journal of Agriculture and Crops (Vol.2, No. 10)Publication Date: 2016-10-15
Authors : Hulya Ozturk Dogan; Mustafa Erat;
Page : 94-100
Keywords : Wheat; Glutathione S-transferase; Purification; Kinetic.;
- Some Kinetic Properties and Inhibition of Glutathione S-Transferase from a Hybridized Wheat (Triticum aestivum L.)
- The Kinectics and Inhibition Properties of Partially Purified Glutathione Transferase from the Head of Palm Weevil (Rynchophorus Phoenicis) Larva
- Stimulatory Effect of Thidiazuron Manipulation on Regeneration of Mature Caryopsis Culture in Bread Wheat (Triticum aestivum L.) and Pasta Wheat (Triticum durum L.)
- Impact of Municipal Waste Leachate Application on Soil Properties and Accumulation of Heavy Metals in Wheat (Triticum aestivum L)
- Effect of Subsoil Compaction Constraints on Some Morphological, Physiological and Agronomic Properties of Wheat (Triticum Aestivum L.) Under Rain-Fed Farming
Abstract
Glutathione S-transferase enzymes (GSTs) play central roles in phase II detoxification of both xenobiotics and endogenous compounds in almost all living organisms. The enzyme was extracted and partially purified from wheat leaves through a procedure including ammonium sulfate fractionation followed by dialysis and gel filtration chromatography. These procedures yielded a 7.14-fold purification with 71% recovery. Optimum activity conditions-pH, temperature and ionic strength-of the enzyme were determined. Its some kinetic properties such as Vmax, KM, and kcat were calculated for GSH and CDNB substrates. The kcat/KM values of the enzyme were 603.5 for GSH and 385.3 for CDNB. The native molecular weight of the enzyme was estimated to be 52 kDa based on its mobility in gel filtration column.
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