Marker-Assisted Backcross Screening for Vietnamese Aromatic Rice Lines Derived from TLR7/Khaodawkmali105//TLR7

This research focused on genotyping via SSR molecular marker. Backcrossing populations of the combination of TLR7/KhaoDawkMali105//TLR7 was generated to stabilize aromatic genes of donors into rice varieties as acceptors by using an advanced backcrossing method (ABC) with molecular marker assistance. Fifty BC3F2 rice lines were used to evaluate aroma detection through the sensory test, genotypic analysis using microsatellite markers, agronomic characteristics, and other grain quality traits. Sixteen lines having aromatic gene (fgr) locus were found using marker RG28 as homozygous condition in 50 rice lines. The marker RM223 detected lowest number of fgr locus. Two lines (BC3F4-1-8 and BC3F4-1-48) were identified well-defined target for aroma genes having high yield and good quality traits. Thus, these lines were utilized SSR marker for demonstrating marker-assisted selection (MAS) breeding program.