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Study of enzymatic properties of some local fungal isolates Lattakia in Syria

Journal: Tishreen University Journal , Biological Sciences Series (Vol.40, No. 2)

Publication Date:

Authors : ;

Page : 11-21

Keywords : Fungi; Enzymes; Food residues; Pectinase; Cultivated medium;

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Abstract

Isolation of fungal strains from local agricultural products is importance to produce enzymes and this explains the ability of these microorganisms to grow among containing macromolecular organic compounds. This work serves to isolate some fungal (Aspergillus niger, Geotrichum candidum, Saccharomycopsis fibuligera) and examine some enzymatic activities such as pectinase (Polygalactorunase, Pectin esterase, Indo-Polygalactorunase), Cellulase, Saccharase, β-amylase and Amyloglocosidase in liquid medium containing various carbon sources (Medium M1 Molasses 4 g%ml, M2 beet sugar residue 2%ml with wheat husks powder 3g%ml and M3 citrus sub-products 3g%ml) fermented by the selected fungal strains demonstrates that their biosynthesis potential for producing some important enzymes for use in different areas of applications, where she gave a significant production capacity of some enzymes by type of fungal and medium of culture. The studied fungus gave good results from Pectinase enzymes in three medium but high amounts by Aspergillus niger and Saccharomycopsis fibuligera has given moderate amounts of Polygalacturonase enzymes in medium M1 and M3 and very little in medium M2 and without Indogalacturonase, and that all the isolated fungal didn,t produce Cellulase in medium M1 and it gave a high activities of Cellulase in the middle M2 with Aspergillus niger and Geotrichum candidum medium, and all strains gave Invertase activity in three mediums of convergent values (40-70) activity unit and Invertase activity of A.niger in the Middle M3 override (U = 160), while Aspergillus niger and Saccharomycopsis fibuligera produce high amounts of enzymes of Amylase in three mediums.

Last modified: 2018-12-23 05:09:10