The Influence Study of The Mole Ratio Reactant in Ceftriaxone Sodium Synthesis Against The Yield of The Production

Ceftriaxone is one of the third generations of cephalosporin antibiotics and commercially found as a sodium salt. The market demand for it is still high in recent years, including in Indonesia. However, there is no local production manufacture yet. A high yield of ceftriaxone sodium would be an advantage in industrial scale. Ceftriaxone was synthesized by reacting 7–amino–3–[(2,5–dihydro–6–hydroxy–2–methyl–5–oxo–1,2,4–triazin–3–yl) thiomethyl] cephalosporanic acid (7-ACT) with 2-Mercaptobenzothiazolyl (Z)-2-(2-Aminothiazole-4-yl)-2-Methoxyimino Acetate (MAEM) then with sodium salt in basic condition. The product was generated by solventing-out using acetone. The products were analyzed by HPLC quantitatively and the structure was confirmed using FTIR, MS and NMR. In this research, the variation in the mole ratio of reactants against the yield of product was evaluated. The result showed that the variations in mole ratio reactants affect the yield production. The higher ratio of MAEM would be the higher yield is obtained. The results show that the yield was 72,17% at mole ratio 1:2 which has 99,32% purity. This result could be a consideration in industrial production scale in ceftriaxone sodium preparation.