Antimicrobial and Antioxidant Activities of Four Selected Noncommercial Honeys
Journal: International Journal of Advanced Engineering Research and Science (Vol.5, No. 12)Publication Date: 2018-12-03
Authors : Yener Tekeli Serbay Bucak;
Page : 264-268
Keywords : Honey; DPPH; total phenolic content; β-carotene assay; MIC.;
Abstract
In this study; we investigated the antioxidant activities and antimicrobial effects of some honey samples (Honeydew, Eucalyptus, Petroselinum crispum M. and flower) from different regions of Hatay, Turkey. The antioxidant capacities were evaluated by using folin assay, free radical scavenging (DPPH) assay and β-carotene-linoleic acid emulsion method and the results compared against reference synthetic antioxidants (BHA, BHT). A 96 well minimum inhibitory concentration (MIC) spectrophotometric-based assay is used to assess antimicrobial activity against some gram positive bacteria and gram negative bacteria. Total phenolic content of the honey samples were determined according to the Folin-Ciocalteau procedure and found between 60,58–287,3 mg GAE/kg honey. The characteristic antioxidant activities showed a marked correlation with the total phenolic contents. The honey samples showed low MIC values against three gram (+) and three gram (-) bacteria. Honeydew honey found to be stronger than the other honeys in terms of both antioxidant and antimicrobial potential.
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